Application
Antibodies tested include p-FAK (A-12): (A,B,C) and FAK (C-903): (D,E,F).Application
Antibodies tested include p-FAK (Ser 722)-R: (A,B,C) and FAK (C-903): (D,E,F).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing focal adhesion sites and cytoplasmic localization.Application
Antibodies tested include p-FAK (Tyr 577)-R: (A,B,C) and FAK (C-903): (D,E,F).Application
Western blot analysis of FAK expression in non-transfected: (A) and human FAK transfected: (B) 293T whole cell lysates.Application
Antibodies tested include FAK (C-20): (A) and FAK (C-903): (B).| Product Name | FAK Antibody (C-903) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 903-1052 of FAK of mouse origin |
| Modification Notes | Focal adhesion kinase was initially identified as a major substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus, it has been postulated that FAK may have an important in vivo role in the re-epithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting through G protein-coupled receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FAK p125 of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 903-1052 of FAK of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 903-1052 of FAK of mouse origin |
| Entrez Gene | 5747 |
|---|
Application
Antibodies tested include p-FAK (A-12): (A,B,C) and FAK (C-903): (D,E,F).
Application
Antibodies tested include p-FAK (Ser 722)-R: (A,B,C) and FAK (C-903): (D,E,F).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing focal adhesion sites and cytoplasmic localization.
Application
Antibodies tested include p-FAK (Tyr 577)-R: (A,B,C) and FAK (C-903): (D,E,F).
Application
Western blot analysis of FAK expression in non-transfected: (A) and human FAK transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include FAK (C-20): (A) and FAK (C-903): (B).| Application Notes | recommended for detection of FAK p125 of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
