Application
Western blot analysis of Syntaphilin expression in non-transfected: (A) and human Syntaphilin transfected: (B) 293T whole cell lysates.| Product Name | Syntaphilin Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 221-470 mapping within an internal region of Syntaphilin of human origin |
| Modification Notes | Syntaxins are categorized as proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25, SNAPs (soluble NSF attachment proteins) and Synaptotagmin. Syntaphilin competes with SNAP 25 for Syntaxin 1 binding. By absorbing free Syntaxin 1, Syntaphilin can inhibit the assembly of the SNARE complex, and thereby regulate Synaptic vesicle exocytosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Syntaphilin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 221-470 mapping within an internal region of Syntaphilin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 221-470 mapping within an internal region of Syntaphilin of human origin |
| Entrez Gene | 9751 |
|---|
Application
Western blot analysis of Syntaphilin expression in non-transfected: (A) and human Syntaphilin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Syntaphilin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
