Application
Antibodies tested include p-nestin (Thr 1495): (A,B,C) and nestin (C-20): (D,E,F).Application
Antibodies tested include p-nestin (Thr 1495): (A,B) and nestin (C-20): (C,D).| Product Name | p-nestin Antibody (Thr 1583) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 1583 of nestin of rat origin |
| Modification | Phosphoralated |
| Modification Notes | Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. Nestin is also a component of the dynamic IF network during muscle development where it polymerizes with desmin and vimentin. Nestin co-assembles with vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nm IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells, and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis. Thr 316 is a cdc2-specific phosphorylation site in Nestin. Phosphorylation at this site is increased during mitosis and is used as a marker for cdk5-specific phosphorylation. Cdk5 and cdc2 induces phosphorylation at both residues Thr 316 and Thr 1583. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Thr 1583 phosphorylated nestin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 1583 of nestin of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 1583 of nestin of rat origin |
| Entrez Gene | 10763 |
|---|
Application
Antibodies tested include p-nestin (Thr 1495): (A,B,C) and nestin (C-20): (D,E,F).
Application
Antibodies tested include p-nestin (Thr 1495): (A,B) and nestin (C-20): (C,D).| Application Notes | recommended for detection of Thr 1583 phosphorylated nestin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
