Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cyto-plasmic and membrane localization.Application
Western blot analysis of PLC γ1 phosphorylation expression in untreated (A) and serum-starved (B) 3T3 whole cell lysates.| Product Name | p-PLC γ1 Antibody (Tyr 771) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Tyr 771 phosphorylated PLC γ1 of m, r and h origin by WB, IP and IF |
| Modification | Phosphoralated |
| Modification Notes | Phospholipase C-gamma 1 (PLC g1) is an isozyme of the phosphoinositide-specific PLC family, which occupies a central role in hormonal signal transduction pathways and is a substrate for the epidermal growth factor receptor tyrosine kinase. Following activation of TrkB, PLC g1 is phosphorylated on Tyrosine 783, Tyrosine 771 and Tyrosine 1253. Tyrosine 783 lies just downstream of the kinase domain in a relatively short sequence motif characteristic of the Trk family of protein-tyrosine kinase receptors. The sequence around Tyrosine 783 fits a consensus sequence for binding PLC g1. PLC g1 also forms a complex with TrkB consistent with the possibility that one of the TrkB autophosphorylation sites provides a binding site for the PLC g1 SH2 domains, as is the case for other receptor protein-tyrosine kinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tyr 771 phosphorylated PLC &gamma 1 of m r and h origin by WB IP and IF |
|---|---|
| Tissue Specificity | recommended for detection of Tyr 771 phosphorylated PLC γ1 of m, r and h origin by WB, IP and IF |
| Entrez Gene | 5335 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cyto-plasmic and membrane localization.
Application
Western blot analysis of PLC γ1 phosphorylation expression in untreated (A) and serum-starved (B) 3T3 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
