Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.Application
Western blot analysis of CD68 expression in non-transfected 293T: (A), human CD68 transfected 293T: (B) and THP-1 (C) whole cell lysates.Application
Western blot analysis of CD68 expression in non-transfected 293T: (A), mouse CD68 transfected 293T: (B) and THP-1 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed THP-1 cells showing cytoplasmic and membrane localization.Application
Western blot analysis of CD68 expression in THP-1 whole cell lysate.| Product Name | CD68 Antibody (H-255) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 100-354 of CD68 of human origin |
| Modification Notes | CD68, which is homologous to the mouse antigen macrosialin, belongs to a family of acidic, highly glycosylated lysosomal glycoproteins (LGPs) that includes lamp-1 and lamp-2 (1,2). CD68 is found in cytoplasmic granules and in the cytoplasm of various non-hematopoietic tissues including liver and kidney tubules and glomeruli (3). CD68 is also found, to a lesser extent, on the surface of macrophages, monocytes, neutrophils, basophils and large lymphocytes (4,5). LGPs are major components of lysosomal membranes and may act to protect the membranes from attack by hydrolases (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 100-354 of CD68 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 100-354 of CD68 of human origin |
| Entrez Gene | 968 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of CD68 expression in non-transfected 293T: (A), human CD68 transfected 293T: (B) and THP-1 (C) whole cell lysates.
Application
Western blot analysis of CD68 expression in non-transfected 293T: (A), mouse CD68 transfected 293T: (B) and THP-1 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed THP-1 cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of CD68 expression in THP-1 whole cell lysate.| Application Notes | recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
