Application
Western blot analysis of A-Raf expression in non-transfected: (A) and human A-Raf transfected: (B) 293T whole cell lysates.Application
Western blot analysis of A-Raf expression in non-transfected control (A) and A-Raf transfected (B) HeLa cells. Blot probed with A-Raf (C-20): . α-actinin (H-2): used as specificity and loading control.Application
Western blot analysis of A-Raf expression in K-562 whole cell lysate.| Product Name | A-Raf Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of A-Raf of human origin |
| Modification Notes | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of A-Raf p68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of A-Raf of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of A-Raf of human origin |
| Entrez Gene | 369 |
|---|
Application
Western blot analysis of A-Raf expression in non-transfected: (A) and human A-Raf transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of A-Raf expression in non-transfected control (A) and A-Raf transfected (B) HeLa cells. Blot probed with A-Raf (C-20): . α-actinin (H-2): used as specificity and loading control.
Application
Western blot analysis of A-Raf expression in K-562 whole cell lysate.| Application Notes | recommended for detection of A-Raf p68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
