Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and membrane localization.Application
Western blot analysis of HMG-1 expression in non-transfected: (A) and human HMG-1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of HMG-1 expression in non-transfected 293T: (A), mouse HMG-1 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of HMG-1/2/3 expression in K-562 (A) and MCF7 (B) nuclear extracts.| Product Name | HMG-1/2/3 Antibody (FL-215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-215 representing full length HMG-1 of human origin |
| Modification Notes | High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFkB family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HMG-1, 2 and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-215 representing full length HMG-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-215 representing full length HMG-1 of human origin |
| Entrez Gene | 3146 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and membrane localization.
Application
Western blot analysis of HMG-1 expression in non-transfected: (A) and human HMG-1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of HMG-1 expression in non-transfected 293T: (A), mouse HMG-1 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of HMG-1/2/3 expression in K-562 (A) and MCF7 (B) nuclear extracts.| Application Notes | recommended for detection of HMG-1, 2 and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
