Application
Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.Application
Western blot analysis of Raf-B expression in PC-12 whole cell lysate.| Product Name | Raf-B Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Raf-B of human origin |
| Modification Notes | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Raf-B of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Raf-B of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Raf-B of human origin |
| Entrez Gene | 673 |
|---|
Application
Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.
Application
Western blot analysis of Raf-B expression in PC-12 whole cell lysate.| Application Notes | recommended for detection of Raf-B of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
