Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Clathrin LCA expression in JAR whole cell lysate.Application
Western blot analysis of Clathrin LCA expression in JAR whole cell lysate (A) and mouse brain tissue extract (B).| Product Name | Clathrin LCA Antibody (H-55) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 51-105 mapping within an internal region of Clathrin LCA of human origin |
| Modification Notes | Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are engulfed by clathrin-coated vesicles. Clathrin is composed of three heavy chains and three light chains which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. Although LCA and LCB are encoded by two discrete genes sharing only 60% homology, they have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms. Additionally, in the brain, LCA and LCB contain inserted sequences that form higher molecular weight isoforms. These sequences insert at similar cytoplasmic domain encoding regions for both LCA and LCB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Clathrin light chain A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 51-105 mapping within an internal region of Clathrin LCA of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 51-105 mapping within an internal region of Clathrin LCA of human origin |
| Entrez Gene | 1211 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Clathrin LCA expression in JAR whole cell lysate.
Application
Western blot analysis of Clathrin LCA expression in JAR whole cell lysate (A) and mouse brain tissue extract (B).| Application Notes | recommended for detection of Clathrin light chain A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
