Application
Western blot analysis of MafG expression in non-transfected: (A) and mouse MafG transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MafF/G/K expression in non-transfected: (A) and mouse MafF/G/K transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NF-E2 p18 (MafK) expression in non-transfected: (A) and mouse NF-E2 p18 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.Application
Western blot analysis of MafF/G/K expression in K-562 (A) and Sol8 (B) nuclear extracts.| Product Name | MafF/G/K Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 63-126 of MafG of human origin |
| Modification Notes | Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. c-Maf (Maf-2) and the closely related family members Neural retina leucine zipper (Nrl), L-Maf, and Krml1/MafB (Maf-1) all bind to T-MARE sites and are implicated in a wide variety of developmental and physiologic roles. The three small Maf family proteins MafF, MafG, and MafK are components of NF-E2 which function as heterodimers with the large tissue-restricted subunit of NF-E2 called p45, and furthermore are implicated in the transcriptional regulation of many erythroid-specific genes. MafG is ubiquitously expressed, with highest expression in the VMS, heart and skeletal muscle; its expression is induced in response to stress. MafK, also designated NF-E2 p18, is primarily expressed during development in mesenchymal and hematopoietic cells and neurons. MafK heterodimerizes with NF-E2 and various CNC proteins. MafF is most abundantly expressed in the lung and is thought to compensate for loss of function mutations in MafG and MafK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of MafF, MafG and NF-E2 p18 (also designated MafK) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 63-126 of MafG of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 63-126 of MafG of human origin |
| Entrez Gene | 4097 |
|---|
Application
Western blot analysis of MafG expression in non-transfected: (A) and mouse MafG transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MafF/G/K expression in non-transfected: (A) and mouse MafF/G/K transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NF-E2 p18 (MafK) expression in non-transfected: (A) and mouse NF-E2 p18 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
Application
Western blot analysis of MafF/G/K expression in K-562 (A) and Sol8 (B) nuclear extracts.| Application Notes | recommended for detection of MafF, MafG and NF-E2 p18 (also designated MafK) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
