Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Laminin α-1 expression in Caki-1 whole cell lysate.| Product Name | Laminin α-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1856-2099 of Laminin α-1 of human origin |
| Modification Notes | Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each laminin is a heterotrimer of a, b and y chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble and bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan and cognate laminin receptors. The human Laminin a-1 gene maps to chromosome 18p11.3 and is over-expressed in Alzheimer disease frontal cortex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Laminin α-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1856-2099 of Laminin &alpha -1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1856-2099 of Laminin α-1 of human origin |
| Entrez Gene | 284217 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Laminin α-1 expression in Caki-1 whole cell lysate.| Application Notes | recommended for detection of Laminin α-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
