Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic and weak nuclear staining of glandular cells.Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic and membrane staining.Application
Antibodies tested include Raf-B (H-145): (A-C) and Raf-B (F-7): (D-F).| Product Name | Raf-B Antibody (H-145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 12-156 mapping at the N-terminus of Raf-B of human origin |
| Modification Notes | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Raf-B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 12-156 mapping at the N-terminus of Raf-B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 12-156 mapping at the N-terminus of Raf-B of human origin |
| Entrez Gene | 673 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic and weak nuclear staining of glandular cells.
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic and membrane staining.
Application
Antibodies tested include Raf-B (H-145): (A-C) and Raf-B (F-7): (D-F).| Application Notes | recommended for detection of Raf-B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
