Application
Western blot analysis of human recombinant MMP-3 fusion protein.| Product Name | MMP-3/10 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 178-477 mapping at the C-terminus of MMP-3 of human origin |
| Modification Notes | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, -2 and -3 respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MMP-3 and MMP-10 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with other MMP family members: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 178-477 mapping at the C-terminus of MMP-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 178-477 mapping at the C-terminus of MMP-3 of human origin |
| Entrez Gene | 4314 |
|---|
Application
Western blot analysis of human recombinant MMP-3 fusion protein.| Application Notes | recommended for detection of MMP-3 and MMP-10 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with other MMP family members: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
