Application
Antibodies tested included NFκB p65 (A): , ATF-2 (N-96): , GCN5 (N-18): , Pol II (H-224): , TFIID (TBP)(SI-1): and CBP (A-22): . Data kindly provided by G. Mosialos.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.Application
Western blot analysis of Pol II expression in A-431 (A), U-2 0S (B) and HeLa (C) whole cell lysates.| Product Name | Pol II Antibody (H-224) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-224 of RNA Pol II of human origin |
| Modification Notes | RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-224 of RNA Pol II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-224 of RNA Pol II of human origin |
| Entrez Gene | 5430 |
|---|
Application
Antibodies tested included NFκB p65 (A): , ATF-2 (N-96): , GCN5 (N-18): , Pol II (H-224): , TFIID (TBP)(SI-1): and CBP (A-22): . Data kindly provided by G. Mosialos.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.
Application
Western blot analysis of Pol II expression in A-431 (A), U-2 0S (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
