Application
Antibodies tested include SIRP-α/β1 (H-300): (A) and SIRP-α/β1 (N-19): (B).| Product Name | SIRP-α/β1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of SIRP-α (signal-regulatory protein-α) of human origin |
| Modification Notes | SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domain-containing protein-tyrosine phosphatase SHP-2 in response to insulin. The SIRP family negatively regulates the PI 3-kinase pathway, which may diminish EGFR-mediated motility and survival phenotypes that contribute to transformation of certain cell types. SIRP-å1 is a transmembrane protein which contains an extracellular portion with three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. SIRP-å (also known as SIRP-å1, SIRP-å2 or SIRP-å3) is a substrate for activated receptor tyrosine kinases. In its tyrosine phosphorylated form, SIRP-å binds to SH-PTP2 through SH2 interactions and acts as an SH-PTP2 substrate. SIRP-å has been shown to have negative regulatory effects on cellular responses induced by growth factors, oncogenes and insulin. SIRP-∫1 shares extensive sequence homology with SIRP-å in its extracellular portion but lacks the cytoplasmic portion. SIRP-©, originally designated SIRP-∫2 (SIRP-B2, CD172g) has unique characteristics from both the å and ∫ versions. SIRP-© is expressed on the majority of T cells and a proportion of B cells. CD47 associates with SIRP-©, and this interaction signals unidirectionally only. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SIRP-α and SIRP-β family members of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of SIRP-&alpha (signal-regulatory protein-&alpha ) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of SIRP-α (signal-regulatory protein-α) of human origin |
| Entrez Gene | 140885 |
|---|
Application
Antibodies tested include SIRP-α/β1 (H-300): (A) and SIRP-α/β1 (N-19): (B).| Application Notes | recommended for detection of SIRP-α and SIRP-β family members of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
