Application
Western blot analysis of GIPC expression in non-transfected: (A) and mouse GIPC transfected: (B) 293T whole cell lysates.| Product Name | GIPC Antibody (H-55) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-55 mapping at the N-terminus GIPC of human origin |
| Modification Notes | GIPC, for GAIP interacting protein at the C terminus (also designated SEMCAP-1 or synectin), is a PDZ domain containing protein that interacts with RGS-GAIP, a GTPase-activating protein (GAP) for G(a i) subunits. GIPC was also identified as TIP-2, a protein that interacts with the viral oncoprotein TAX, which transactivates viral and cellular promoters through interactions with various transcription factors. PDZ domain-containing proteins are primarily localized to cell-cell junctions in epithelial cells and neurons where they coordinate the assembly of multiprotein complexes. GIPC specifically localizes to clusters of vesicles near the plasma membrane and participates in G protein-coupled signaling pathway involved in regulating clathrin-coated vesicular trafficking. GIPC also associates with membrane bound semaphorin F (M-SemF), which is involved in neuronal axon growth, and it appears to regulate the subcellular distribution of M-SemF in the brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GIPC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-55 mapping at the N-terminus GIPC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-55 mapping at the N-terminus GIPC of human origin |
| Entrez Gene | 10755 |
|---|
Application
Western blot analysis of GIPC expression in non-transfected: (A) and mouse GIPC transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of GIPC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
