Application
Western blot analysis of p130 Cas expression in non-transfected: (A) and human p130 Cas transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Sin expression in non-transfected: (A) and mouse Sin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Sin expression in Jurkat whole cell lysate.Application
Immunofluorescence staining of methanol-fixed 3611-RF cells showing cytoplasmic staining.Application
Western blot analysis of p130 Cas expression in 3611-RF whole cell lysate.| Product Name | p130 Cas/Sin Antibody (N-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of p130 Cas of rat origin |
| Modification Notes | A protein designated p130 Cas (for Crk-associated substrate), represents one of several known substrates for v-Crk encoded p47. p130 Cas (also designated breast cancer anti-estrogen resistance protein 1 or Cas scaffolding protein family member 1), exhibits a high level of tyrosine phosphorylation and is tightly associated with v-Crk, suggesting a role in v-Crk-mediated cell signaling. p130 Cas is a novel SH3-containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. Two p130 Cas related proteins, designated Sin (Src interacting or signal integrating protein, also designated Cas3 or HEFS) and Cas-L (human enhancer of filamentatin 1, HEF1 or Cas3), have also been identified. Sin contains SH2/SH3 domains and has been shown to activate Src. Cas-L contains an SH3 domain and has been shown to be a docking protein that serves as a substrate for phosphorylation by several oncogenic tyrosine kinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p130 Cas and Sin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of p130 Cas of rat origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of p130 Cas of rat origin |
| Entrez Gene | 9564 |
|---|
Application
Western blot analysis of p130 Cas expression in non-transfected: (A) and human p130 Cas transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Sin expression in non-transfected: (A) and mouse Sin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Sin expression in Jurkat whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed 3611-RF cells showing cytoplasmic staining.
Application
Western blot analysis of p130 Cas expression in 3611-RF whole cell lysate.| Application Notes | recommended for detection of p130 Cas and Sin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
