Application
Western blot analysis of VCAM-1 expression in non-transfected: (A) and human VCAM-1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of purified human VCAM-1 immunoprecipitated with VCAM-1 (P8M1): and detected with VCAM-1 (H-276): .Application
Western blot analysis of purified human VCAM-1 immunoprecipitated with VCAM-1 (P3C4): and detected with VCAM-1 (H-276): .Application
Western blot analysis of purified human VCAM-1 immunoprecipitated with VCAM-1 (M/K-2): and detected with VCAM-1 (H-276): .Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil showing membrane staining.Application
Western blot analysis of human recombinant VCAM-1 fusion protein.| Product Name | VCAM-1 Antibody (H-276) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 25-300 mapping within the N-terminus of VCAM-1 of human origin |
| Modification Notes | Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms’ tumors, Ewing’s sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2å1 and B2åM integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM) -like protein and is deleted in 66% of patients with Kallmann's syndrome, anosmia with secondary hypogonadism . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of VCAM-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 25-300 mapping within the N-terminus of VCAM-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 25-300 mapping within the N-terminus of VCAM-1 of human origin |
| Entrez Gene | 7412 |
|---|
Application
Western blot analysis of VCAM-1 expression in non-transfected: (A) and human VCAM-1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of purified human VCAM-1 immunoprecipitated with VCAM-1 (P8M1): and detected with VCAM-1 (H-276): .
Application
Western blot analysis of purified human VCAM-1 immunoprecipitated with VCAM-1 (P3C4): and detected with VCAM-1 (H-276): .
Application
Western blot analysis of purified human VCAM-1 immunoprecipitated with VCAM-1 (M/K-2): and detected with VCAM-1 (H-276): .
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil showing membrane staining.
Application
Western blot analysis of human recombinant VCAM-1 fusion protein.| Application Notes | recommended for detection of VCAM-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
