Application
Western blot analysis of Shf expression in non-transfected 293T: (A), truncated mouse Shf transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Shb/Shf expression in K-562 whole cell lysate.| Product Name | Shb/Shf Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Shb of human origin |
| Modification Notes | The SH2 (Src Homology 2) domain is a structurally conserved motif that contains two alpha helices and seven beta strands and is found in a variety of proteins that are involved in signal transduction throughout the cell. Specifically, the SH2 domain targets SH2 domain-containing proteins to tyrosine-phosphorylated sites, an event that can trigger a protein-protein interaction cascade which may ultimately effect gene expression and cellular function. Shb (SH2 domain-containing adapter protein b), Shd (SH2 domain-containing adapter protein d), She (SH2 domain-containing adapter protein e) and Shf (SH2 domain-containing adapter protein f) are SH2 domain-containing proteins that play various roles throughout the cell. Shb is a widely expressed protein that localizes to both the cell membrane and the cytoplasm and plays an important role in signal transduction, mainly by linking activated proteins to downstream signaling targets, thereby propagating a signal cascade. Unlike Shb, Shd and Shf are thought to function as adaptor proteins, the former of which may be involved in apoptotic regulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Shb, Shf, and to a lesser extent, Shd and She of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Shb of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Shb of human origin |
| Entrez Gene | 6461 |
|---|
Application
Western blot analysis of Shf expression in non-transfected 293T: (A), truncated mouse Shf transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Shb/Shf expression in K-562 whole cell lysate.| Application Notes | recommended for detection of Shb, Shf, and to a lesser extent, Shd and She of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
