Application
Western blot analysis of ICOS expression in U-698-M (A) and HL-60 (B) whole cell lysates.Application
Western blot analysis of ICOS expression in Jurkat (A), THP-1 (B), U-698-M (C) and HL-60 (D) whole cell lysates.Application
Western blot analysis of ICOS expression in BYDP (A), NIH/3T3 (B) and RAW 264.7 (C) whole cell lysates and mouse lymph node (D) and mouse spleen (E) tissue extracts.Application
Western blot analysis of ICOS expression in non-transfected: (A) and human ICOS transfected: (B) 293T whole cell lysates.| Product Name | ICOS Antibody (FL-199) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-199 representing full length ICOS of human origin |
| Modification Notes | T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells. CD80 and CD86 bind to the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. ICOS (inducible co-stimulator) is related to CD28 and CTLA-4, and these three proteins are thought to compose a receptor family. ICOS stimulation enhances T cell responses and superinduces the synthesis of IL-10, but it does not induce IL-2 upregulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ICOS of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-199 representing full length ICOS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-199 representing full length ICOS of human origin |
| Entrez Gene | 29851 |
|---|
Application
Western blot analysis of ICOS expression in U-698-M (A) and HL-60 (B) whole cell lysates.
Application
Western blot analysis of ICOS expression in Jurkat (A), THP-1 (B), U-698-M (C) and HL-60 (D) whole cell lysates.
Application
Western blot analysis of ICOS expression in BYDP (A), NIH/3T3 (B) and RAW 264.7 (C) whole cell lysates and mouse lymph node (D) and mouse spleen (E) tissue extracts.
Application
Western blot analysis of ICOS expression in non-transfected: (A) and human ICOS transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ICOS of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
