Application
Western blot analysis of human truncated recombinant Ret fusion protein.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Antibodies tested include Ret (C-20): (A,B) and Ret (C-19): (C,D).Application
Western blot analysis of Ret expression in Ret isoform A (A) and Ret isoform C (B) transfected NIH/3T3 cells.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing membrane localization.| Product Name | Ret Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Ret isoform C of human origin |
| Modification Notes | The Ret proto-oncogene is structurally related to the growing family of tyrosine kinase transmembrane receptors and is involved in GDNF signaling. By alternative splicing, two isoforms of the Ret proto-oncogene product are generated. The isoforms differ from each other by having either 9 or 51 carboxy terminal amino acids. The Ret gene products include two glycosylated proteins and, in tunicamycin treated cells, a non-glycosylated protein consistent with the predicted Ret molecular weight based on sequence analysis. Tumor-specific rearrangements of the Ret proto-oncogene have been identified in papillary thyroid carcinomas leading to the formation of different transforming fusion proteins sharing the tyrosine kinase domain of Ret. In contrast to the Ret proto-oncogene, the rearranged forms are constitutively phosphorylated on tyrosine and are translocated from the membrane to the cytoplasm. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ret isoform C and, to a lesser extent, Ret isoform A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Ret isoform C of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Ret isoform C of human origin |
| Entrez Gene | 5979 |
|---|
Application
Western blot analysis of human truncated recombinant Ret fusion protein.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Antibodies tested include Ret (C-20): (A,B) and Ret (C-19): (C,D).
Application
Western blot analysis of Ret expression in Ret isoform A (A) and Ret isoform C (B) transfected NIH/3T3 cells.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing membrane localization.| Application Notes | recommended for detection of Ret isoform C and, to a lesser extent, Ret isoform A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
