Application
Antibodies tested include p-c-Abl/Arg (Tyr 393/439): (A,B) and c-Abl (K-12): (C,D).Application
Antibodies tested include p-c-Abl (7.Tyr 412): (A,B) and c-Abl (K-12): (C,D).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in Gloeruli and Tubules.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of c-Abl expression in K-562 whole cell lysate.| Product Name | c-Abl Antibody (K-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the kinase domain of c-Abl of human origin |
| Modification Notes | The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia (CML), 20-25% of adult acute lymphoblastic leukemia (ALL) and 2-5% of pediatric ALL. In these leukemias the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing the Philadelphia (Ph1) chromosome. The molecular consequence of this trans-location is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein-tyrosine kinase. The Bcr gene has been shown to encode a GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, p21rac. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of c-Abl and chimeric Bcr/Abl proteins of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the kinase domain of c-Abl of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the kinase domain of c-Abl of human origin |
| Entrez Gene | 25 |
|---|
Application
Antibodies tested include p-c-Abl/Arg (Tyr 393/439): (A,B) and c-Abl (K-12): (C,D).
Application
Antibodies tested include p-c-Abl (7.Tyr 412): (A,B) and c-Abl (K-12): (C,D).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in Gloeruli and Tubules.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of c-Abl expression in K-562 whole cell lysate.| Application Notes | recommended for detection of c-Abl and chimeric Bcr/Abl proteins of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
