Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of NF-YB expression in RAW 264.7 nuclear extract.| Product Name | NF-YB Antibody (FL-207) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-207 representing full length NF-YB of human origin |
| Modification Notes | The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukar-yotic histones. The DNA-binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein, histone-fold “handshake” motif in a manner analogous to the histone proteins H2B and H2A, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of NF-YB of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-207 representing full length NF-YB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-207 representing full length NF-YB of human origin |
| Entrez Gene | 4801 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of NF-YB expression in RAW 264.7 nuclear extract.| Application Notes | recommended for detection of NF-YB of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
