Application
Western blot analysis of CD2AP expression in A-431 (A) and U-2 OS (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.| Product Name | CD2AP Antibody (H-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 350-639 mapping at the C-terminus of CD2AP of human origin |
| Modification Notes | CD2-associated protein (CD2AP) is a cytosolic adaptor molecule that complexes with the intracellular portion of CD2 upon T cell activation. T cell activation induces cell adhesion through CD2-mediated binding to surface ligands on antigen-presenting cells, which enhances antigen-specific T cell activation, potentiates cell clustering and induces cytoskeletal polarization. CD2AP is expressed at highest levels in liver, thymus and spleen. CD2AP contains three SH3 domains that are essential for the interaction with CD2. Mutations in CD2AP that impair this interaction result in the disruption of cell clustering and polarization in activated T lymphocytes. Mice deficient in CD2AP develop a lethal congenital nephrotic syndrome, indicating that CD2AP is also involved in maintaining the integrity of the renal glomerulus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD2AP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 350-639 mapping at the C-terminus of CD2AP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 350-639 mapping at the C-terminus of CD2AP of human origin |
| Entrez Gene | 23607 |
|---|
Application
Western blot analysis of CD2AP expression in A-431 (A) and U-2 OS (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.| Application Notes | recommended for detection of CD2AP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
