Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing membrane and cytoplasmic staining of cells in tubules.Application
Western blot analysis of DAT expression in BE (2)-M17 (A), IMR-32 (B), SK-N-SH (C), SH-SY5Y (D), SK-N-MC (E) and EOC 20 (F) whole cell lysates.Application
Western blot analysis of DAT expression in SH-SY5Y (A) and SK-N-SH (B) whole cell lysates and mouse kidney (C) and mouse brain (D) tissue extracts.| Product Name | DAT Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 541-620 mapping at the C-terminus of the sodium-dependent dopamine transporter (DAT) of human origin |
| Modification Notes | The members of the G protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory, depending on the type of receptor to which it binds. β-adrenergic receptor bound to adrenaline activates adenylyl cyclase, while α2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. The dopamine receptors are divided into two classes, D1 and D2, which differ in their functional characteristics in that D1 receptors stimulate adenylyl cyclase, while D2 receptors inhibit adenylyl cyclase activity. Five different subtypes of dopamine receptor have been described to date. D1DR and D5DR belong to the D1 subclass, while D2DR, D3DR and D4DR belong to the D2 subclass of dopamine receptors. The dopamine transporter, DAT, is a sodium and chloride-dependent dopamine transporter. DAT also can transport dopamine neurotoxins and has been implicated in the selective vulnerability of nigrostriatal dopaminergic neurons in major models of Parkinson’s disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of dopamine transporter of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 541-620 mapping at the C-terminus of the sodium-dependent dopamine transporter (DAT) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 541-620 mapping at the C-terminus of the sodium-dependent dopamine transporter (DAT) of human origin |
| Entrez Gene | 6531 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing membrane and cytoplasmic staining of cells in tubules.
Application
Western blot analysis of DAT expression in BE (2)-M17 (A), IMR-32 (B), SK-N-SH (C), SH-SY5Y (D), SK-N-MC (E) and EOC 20 (F) whole cell lysates.
Application
Western blot analysis of DAT expression in SH-SY5Y (A) and SK-N-SH (B) whole cell lysates and mouse kidney (C) and mouse brain (D) tissue extracts.| Application Notes | recommended for detection of dopamine transporter of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
