Application
Western blot analysis of HNF-4α expression in non-transfected 293T: (A), mouse HNF-4α transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of HNF-4α expression in Hep G2 whole cell lysate.| Product Name | HNF-4α Antibody (H-171) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 295-465 mapping at the C-terminus of HNF-4α of human origin |
| Modification Notes | HNF-1 (å and ∫), HNF-3 (å, ∫ and ©), HNF-4 (å and ©) and HNF-6 compose, in part, a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3å, HNF-3∫ and HNF-3© regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3å and HNF-3∫ have also been shown to be involved in gastrulation events such as body axis formation. HNF-4å and HNF-4© have been shown to be important for early embryo development. HNF-4å is expressed in liver, kidney, pancreas, small intestine, testis and colon; and HNF-4© is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3∫, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and å-1 antitrypsin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of HNF-4α and, to a lesser extent, HNF-4γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 295-465 mapping at the C-terminus of HNF-4&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 295-465 mapping at the C-terminus of HNF-4α of human origin |
| Entrez Gene | 3172 |
|---|
Application
Western blot analysis of HNF-4α expression in non-transfected 293T: (A), mouse HNF-4α transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of HNF-4α expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of HNF-4α and, to a lesser extent, HNF-4γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
