| Product Name | BDNF Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of BDNF of human origin |
| Modification Notes | Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature BDNF and, to a lesser extent, NGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA : WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC-p:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, canine, bovine, porcine, avian and feline |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of BDNF of human origin |
|---|---|
| Molecular Weight(MW) | 15 kDa |
| Tissue Specificity | epitope mapping within an internal region of BDNF of human origin |
| Entrez Gene | 627 |
|---|
| Application Notes | recommended for detection of precursor and mature BDNF and, to a lesser extent, NGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA : WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC-p:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
