Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans cells.Application
Western blot analysis of Clusterin expression in non-transfected: (A) and human Clusterin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Clusterin expression in non-transfected: (A) and human Clusterin transfected: (B) 293T whole cell lysates.| Product Name | Clusterin Antibody (H-330) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 120-449 mapping at the C-terminus of Clusterin of human origin |
| Modification Notes | Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (ApoJ), sulfated glycoprotein 2 (SGP2), SP-40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, å and ∫, are associated through disulfide bonds. The ∫ subunit (also called ApoJå) corresponds to residues 23-227. The å subunit (also called ApoJ∫) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences ∫-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer’s disease pathogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Clusterin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 120-449 mapping at the C-terminus of Clusterin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 120-449 mapping at the C-terminus of Clusterin of human origin |
| Entrez Gene | 1191 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans cells.
Application
Western blot analysis of Clusterin expression in non-transfected: (A) and human Clusterin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Clusterin expression in non-transfected: (A) and human Clusterin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Clusterin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
