Application
Western blot analysis of human recombinant DBH fusion protein.Application
Western blot analysis of DBH expression in non-transfected: (A) and human DBH transfected: (B) 293T whole cell lysates.| Product Name | DBH Antibody (H-213) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 391-603 mapping at the C-terminus of dopamine beta hydroxylase of human origin |
| Modification Notes | Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to noradrenaline in the biosynthesis of catecholamines. DBH is selectively expressed in noradrenergic and adrenergic neurons, as well as in neuroendocrine cells, and it serves as a specific protein marker for noradrenergic processes. The active form of DBH is a homotetramer, which is found in the lumen of synaptic vesicles of corresponding nerve cells, where it localizes to both the membrane and cytosol. DBH is induced by nerve growth factor and insulin growth factor-1 and is regulated by intracellular second messengers protein kinase A, cyclic AMP, diacyl glycerol and Ca2+. Expression of DBH is transcriptionally mediated by Sp1, CREB and AP-1 proteins including c-Fos, c-Jun and JunD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of dopamine beta hydroxylase (DBH) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 391-603 mapping at the C-terminus of dopamine beta hydroxylase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 391-603 mapping at the C-terminus of dopamine beta hydroxylase of human origin |
| Entrez Gene | 1621 |
|---|
Application
Western blot analysis of human recombinant DBH fusion protein.
Application
Western blot analysis of DBH expression in non-transfected: (A) and human DBH transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of dopamine beta hydroxylase (DBH) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
