Application
Western blot analysis of St3Gal-IV expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing cytoplasmic localization.Application
Western blot analysis of St3Gal-IV expression in HeLa whole cell lysate.| Product Name | St3Gal-IV Antibody (P-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of St3Gal-IV of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Cell type-specific expression of unique carbohydrate structures on cell surface glycoproteins and glycolipids provides information relevant to cell-cell interactions in developing and adult organisms. Sialyltransferases contribute to the diversity of carbohydrate structures through their attachment of sialic acid in various terminal positions on glycolipid and on glycoprotein (N-linked and O-linked) carbohydrate groups. The alpha2,3 sialyltransferase (ST3Gal-IV), also known as SIAT4-C and SI4C, shows elevated expression in brain tissues. Sialyltransferase genes are dispersed throughout the human genome. The human SIAT4C gene maps to human chromosome 11q24.2 and encodes St3Gal-IV. Multiple ST3Gal sialyltransferases, including St3Gal-IV, contribute to selectin ligand formation. Selectin ligands are glycan structures that participate in leukocyte trafficking and inflammation. ST3Gal IV expression is down-regulated in human renal cell carcinoma (RCC) and may be one of the factors associated with the malignant progression of human RCC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of St3Gal-IV of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of St3Gal-IV of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 6484 |
|---|
Application
Western blot analysis of St3Gal-IV expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing cytoplasmic localization.
Application
Western blot analysis of St3Gal-IV expression in HeLa whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
