Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane localization.Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic and membrane localization.Application
Western blot analysis of Neu phosphorylation expression in untreated (A) and EGF treated (B) MDA whole cell lysates.| Product Name | p-Neu Antibody (Tyr 1248) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Tyr 1248 phosphorylated Neu of m, r and h origin by WB, IP, IF and IHC(P) |
| Modification | Phosphoralated |
| Modification Notes | Neu (v-ErbB-2 erythroblastic leukemia viral oncogene homolog 2, HER-2, NGL, TKR1, c-ErbB-2) oncogene was originally cloned from a rat neuroglio-blastoma. Human Neu is referred to as HER2 since the protein structure re-sembles human epidermal growth factor receptor (HER). ErbB-2 refers to a high level of similarity to ErbB (avian erythroblastosis oncogene B), later found to code for EGFR (HER). Tyr 1248 phosphorylated Neu localizes with Mucin4/Sialomucin complex at the apical surfaces of ductal and alveolar cells in rodent lactating gland. Phosphorylation of Neu at Tyr 1139 promotes association of GRB2 and GRB7 through a Src homology 2 (SH2) domain-dependent interaction, and contributes to the etiology of certain breast, gastric and esophageal cancers, and testicular germ cell tumors. Neu phosphorylation on Tyr 1221 and Tyr 1248 promotes association of Shc (SH2 domain-containing transforming protein 1) through an SH2 domain. Neu phosphorylation at Tyr 1196 and Tyr 1248 promotes association of Shc through a PTB (phosphotyrosine binding) domain. SH2 and PTB domains recognize tyrosine phosphorylated proteins in a sequence-specific fashion and transduce extracellular signals via subcellular targeting, directing assembly of complexes and modulating enzymatic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tyr 1248 phosphorylated Neu of m r and h origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Tyr 1248 phosphorylated Neu of m, r and h origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 2064 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane localization.
Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of Neu phosphorylation expression in untreated (A) and EGF treated (B) MDA whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
