Application
Western blot analysis of mGluR-1a/5 expression in mouse brain tissue extract.Application
Western blot analysis of mGluR-1a/5 expression in rat mGluR-5 (A) and rat mGluR-1a (B) transfected 293 whole cell lysates.| Product Name | mGluR-1a/5 Antibody (G-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of mGluR-1a/5 of mouse, rat and human origin by WB, IP and IF |
| Modification Notes | The mGluR proteins (metabotropic glutamate receptors) are members of the G-protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic glutamate receptors). The function of mGluR proteins are mediated by two G-proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR-1 and mGluR-5, both of which act as a receptor for glutamate, are mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. mGluR-1 localizes to the cell membrane where it exists as a homodimer composed of two alternatively spliced isoforms, designated mGluR-1a and mGluR-1b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of mGluR-1a/5 of mouse rat and human origin by WB IP and IF |
|---|---|
| Tissue Specificity | recommended for detection of mGluR-1a/5 of mouse, rat and human origin by WB, IP and IF |
| Entrez Gene | 2911 |
|---|
Application
Western blot analysis of mGluR-1a/5 expression in mouse brain tissue extract.
Application
Western blot analysis of mGluR-1a/5 expression in rat mGluR-5 (A) and rat mGluR-1a (B) transfected 293 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
