Application
Western blot analysis of ZO-1 expression in non-transfected: (A) and human ZO-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ZO-1 expression in non-transfected: (A) and human ZO-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ZO-1 expression in MDCK whole cell lysate.Application
Western blot analysis of ZO-1 expression in rat testis tissue extract.WB
(D) Western blotting results of Occludin, ZO-1, and Lyz1 protein expression in the ileum.| Product Name | ZO-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane-associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3 These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of ZO-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1437-1736 mapping at the C-terminus of ZO-1 (Zona occludens-1) of human origin |
|---|---|
| Molecular Weight(MW) | 195KD |
| Tissue Specificity | epitope corresponding to amino acids 1437-1736 mapping at the C-terminus of ZO-1 (Zona occludens-1) of human origin |
| Entrez Gene | 7082 |
|---|
Application
Western blot analysis of ZO-1 expression in non-transfected: (A) and human ZO-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ZO-1 expression in non-transfected: (A) and human ZO-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ZO-1 expression in MDCK whole cell lysate.
Application
Western blot analysis of ZO-1 expression in rat testis tissue extract.
WB
(D) Western blotting results of Occludin, ZO-1, and Lyz1 protein expression in the ileum.| Application Notes | recommended for detection of ZO-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
