Application
Western blot analysis of NF-YA expression in non-transfected: (A) and mouse NF-YA transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.Application
Western blot analysis of NF-YA expression in A-431 (A) and K-562 (B) whole cell lysates.| Product Name | NF-YA Antibody (H-209) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 139-347 of CCAAT-binding transcription factor subunit B (NF-YA) of human origin |
| Modification Notes | The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB, and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold “handshake” motif in a manner analogous to the histone proteins, H2B and H2A, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of NF-YA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 139-347 of CCAAT-binding transcription factor subunit B (NF-YA) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 139-347 of CCAAT-binding transcription factor subunit B (NF-YA) of human origin |
| Entrez Gene | 4800 |
|---|
Application
Western blot analysis of NF-YA expression in non-transfected: (A) and mouse NF-YA transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.
Application
Western blot analysis of NF-YA expression in A-431 (A) and K-562 (B) whole cell lysates.| Application Notes | recommended for detection of NF-YA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
