Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of IRS-2 expression in HeLa (A) and 3T3-L1 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed 3T3-L1 cells showing cytoplasmic staining.| Product Name | IRS-2 Antibody (H-205) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 926-1130 of IRS-2 of human origin |
| Modification Notes | IRS-2, originally described as 4PS, acts as a signaling intermediate downstream of the insulin, IGF-1, IL-4, IL-9 and IL-13 receptors (1). In IRS-2-deficient mice, reduction in total PI 3-kinase activity by 30% and abolition of downstream activation of protein kinase C (PKC) zeta leads to the development of type 2 diabetes (3). Additionally, reconstitution with retroviral IRS-2 restores IRS-2/PI 3-kinase/PKC zeta signalling as well as glucose uptake (3). IRS-2 translocates to the nuclei of mouse embryo fibroblasts expressing the insulin-like growth factor 1 receptor (4). Various mutations in the IGF-IR can result in an abrogation of or decrease in the translocation of IRS proteins to the nucleoli (4). IRS-2 is responsible for mitogen-activated protein kinase (MAPK) and protein kinase B (PKB) activation by insulin and is the major adapter molecule linking the insulin receptor to this step (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IRS-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 926-1130 of IRS-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 926-1130 of IRS-2 of human origin |
| Entrez Gene | 8660 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of IRS-2 expression in HeLa (A) and 3T3-L1 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed 3T3-L1 cells showing cytoplasmic staining.| Application Notes | recommended for detection of IRS-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
