Application
Immunofluorescence staining of methanol-fixed C32 cells showing nuclear localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.Application
Western blot analysis of cyclin D1 expression in RAW 264.7 whole cell lysate.Application
Western blot analysis of cyclin D1 expression in non-transfected: (A) and human cyclin D1 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include cyclin D1 (H-295): (A,B) and cyclin D1 (M-20): (C,D).| Product Name | cyclin D1 Antibody (H-295) FITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-295 representing full length cyclin D1 of human origin |
| Modification Notes | The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also strongly cross-reactive with cyclin D2 and cyclin D3 also reactive with additional species, including canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-295 representing full length cyclin D1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-295 representing full length cyclin D1 of human origin |
| Entrez Gene | 595 |
|---|
Application
Immunofluorescence staining of methanol-fixed C32 cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.
Application
Western blot analysis of cyclin D1 expression in RAW 264.7 whole cell lysate.
Application
Western blot analysis of cyclin D1 expression in non-transfected: (A) and human cyclin D1 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include cyclin D1 (H-295): (A,B) and cyclin D1 (M-20): (C,D).| Application Notes | recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also strongly cross-reactive with cyclin D2 and cyclin D3 also reactive with additional species, including canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
