Application
Western blot analysis of Bad expression in BJAB (A) and MCF7 (B) whole cell lysates and mouse prostate (C) and rat prostate (D) tissue extracts.Application
Antibodies tested include p-Bad (C-10): (A,B,C) and Bad (H-168): (D,E,F).Application
Western blot analysis of Bad phosphorylation in non-transfected: (A,E), untreated human Bad transfected: (B,F), PMA treated human Bad transfected: (C,G) and PMA and lambda protein phosphatase (A) treated human Bad transfected: (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (Ser 112)-R: (A,B,C,D) and Bad (H-168): (E,F,G,H).Application
Western blot analysis of Bad phosphorylation in non-transfected: (A,E), untreated human Bad transfected: (B,F), PMA treated human Bad transfected: (C,G) and PMA and lambda protein phosphatase (A) treated human Bad transfected: (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (C-10): (A,B,C,D) and Bad (H-168): (E,F,G,H).Application
Western blot analysis of Bad expression in non-transfected: (A) and human Bad transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Bad siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Bad (H-168): .Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Antibodies tested include Bad (H-168): (A) and Bad (N-19): (B).| Product Name | Bad Antibody (H-168) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-168 representing full length Bad of human origin |
| Modification Notes | The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2 limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2 and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL: Bax complexes thereby causing restoration of Bax-mediated apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Bad of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-168 representing full length Bad of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-168 representing full length Bad of human origin |
| Entrez Gene | 572 |
|---|
Application
Western blot analysis of Bad expression in BJAB (A) and MCF7 (B) whole cell lysates and mouse prostate (C) and rat prostate (D) tissue extracts.
Application
Antibodies tested include p-Bad (C-10): (A,B,C) and Bad (H-168): (D,E,F).
Application
Western blot analysis of Bad phosphorylation in non-transfected: (A,E), untreated human Bad transfected: (B,F), PMA treated human Bad transfected: (C,G) and PMA and lambda protein phosphatase (A) treated human Bad transfected: (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (Ser 112)-R: (A,B,C,D) and Bad (H-168): (E,F,G,H).
Application
Western blot analysis of Bad phosphorylation in non-transfected: (A,E), untreated human Bad transfected: (B,F), PMA treated human Bad transfected: (C,G) and PMA and lambda protein phosphatase (A) treated human Bad transfected: (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (C-10): (A,B,C,D) and Bad (H-168): (E,F,G,H).
Application
Western blot analysis of Bad expression in non-transfected: (A) and human Bad transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Bad siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Bad (H-168): .
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Antibodies tested include Bad (H-168): (A) and Bad (N-19): (B).| Application Notes | recommended for detection of Bad of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
