Application
Western blot analysis of CRM1 expression in non-transfected 293T: (A), human CRM1 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of CRM1 expression in HeLa (A), A-431 (B), K-562 (C) and Jurkat (D) nuclear extracts.Application
Western blot analysis of CRM1 expression in non-transfected: (A) and mouse CRM1 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining in decidual and trophoblastic cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and CRM1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with CRM1 (H-300): .Application
Western blot analysis of CRM1 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.| Product Name | CRM1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 772-1071 mapping at the C-terminus of CRM1 of human origin |
| Modification Notes | Protein transport across the nucleus is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin alpha1 and karyopherin beta1 subunits. p62 glycoprotein is a nucleoporin that is not only involved in the nuclear import of proteins, but also the export of nascent mRNA strands. NTF2 (nuclear transport factor 2) interacts with nucleoporin p62 as a homodimer composed of two monomers, and may be an obligate component of functional p62. CRM1 has been shown to be an export receptor for leucine-rich proteins that contain the nuclear export signal (NES). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CRM1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 772-1071 mapping at the C-terminus of CRM1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 772-1071 mapping at the C-terminus of CRM1 of human origin |
| Entrez Gene | 7514 |
|---|
Application
Western blot analysis of CRM1 expression in non-transfected 293T: (A), human CRM1 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of CRM1 expression in HeLa (A), A-431 (B), K-562 (C) and Jurkat (D) nuclear extracts.
Application
Western blot analysis of CRM1 expression in non-transfected: (A) and mouse CRM1 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining in decidual and trophoblastic cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and CRM1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with CRM1 (H-300): .
Application
Western blot analysis of CRM1 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.| Application Notes | recommended for detection of CRM1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
