Application
Western blot analysis of cathepsin D expression in non-transfected 293T: (A), human cathepsin D transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of cathepsin D expression in non-transfected 293T: (A), mouse cathepsin D transfected 293T: (B) and MCF7 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of cathepsin D precursor expression in ZR-75-1 (A) and MCF7 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.| Product Name | cathepsin D Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-75 mapping at the N-terminus of cathepsin D of human origin |
| Modification Notes | The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsins may also cleave some protein precursors, thereby releasing regulatory peptides. The promoter region of the cathepsin D gene contains five Sp1 binding sites and four AP-2 binding sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of cathepsin D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-75 mapping at the N-terminus of cathepsin D of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-75 mapping at the N-terminus of cathepsin D of human origin |
| Entrez Gene | 1509 |
|---|
Application
Western blot analysis of cathepsin D expression in non-transfected 293T: (A), human cathepsin D transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of cathepsin D expression in non-transfected 293T: (A), mouse cathepsin D transfected 293T: (B) and MCF7 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of cathepsin D precursor expression in ZR-75-1 (A) and MCF7 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.| Application Notes | recommended for detection of cathepsin D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
