Application
Western blot analysis of LIMK-2 expression in untreated (A) and PMA-treated THP-1 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of LIMK-2 expression in JAR whole cell lysate (A) and mouse placenta extract (B).| Product Name | LIMK-2 Antibody (H-78) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 561-638 mapping at the C-terminus of LIMK-2 of human origin |
| Modification Notes | Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, incuding LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and Cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LIMK-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 561-638 mapping at the C-terminus of LIMK-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 561-638 mapping at the C-terminus of LIMK-2 of human origin |
| Entrez Gene | 3985 |
|---|
Application
Western blot analysis of LIMK-2 expression in untreated (A) and PMA-treated THP-1 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of LIMK-2 expression in JAR whole cell lysate (A) and mouse placenta extract (B).| Application Notes | recommended for detection of LIMK-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
