Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic and nuclear staining of cells in red pulp.Application
Western blot analysis of α-actinin-1 expression in non-transfected: (A) and human α-actinin-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of α-actinin expression in K-562 whole cell lysate.Application
Western blot analysis of α-actinin-4 expression in non-transfected 293T: (A), human α-actinin-4 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of α-actinin expression in 293T (A) and NIH/3T3 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-673 cells showing cytoplasmic staining.Application
Western blot analysis of α-actinin expression in A-673 (A) and SJRH30 (B) whole cell lysates and rat skeletal muscle extract (C).| Product Name | α-actinin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 593-892 mapping at the C-terminus of α-actinin-1 of human origin |
| Modification Notes | The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and å-actinins. There are four tissue-specific å-actinins, namely å-actinin-1, å-actinin-2, å-actinin-3 and å-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each å-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding å-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 593-892 mapping at the C-terminus of &alpha -actinin-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 593-892 mapping at the C-terminus of α-actinin-1 of human origin |
| Entrez Gene | 81 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic and nuclear staining of cells in red pulp.
Application
Western blot analysis of α-actinin-1 expression in non-transfected: (A) and human α-actinin-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of α-actinin expression in K-562 whole cell lysate.
Application
Western blot analysis of α-actinin-4 expression in non-transfected 293T: (A), human α-actinin-4 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of α-actinin expression in 293T (A) and NIH/3T3 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-673 cells showing cytoplasmic staining.
Application
Western blot analysis of α-actinin expression in A-673 (A) and SJRH30 (B) whole cell lysates and rat skeletal muscle extract (C).| Application Notes | recommended for detection of α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
