Application
Western blot analysis of CIS expression in non-transfected: (A) and human CIS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CIS expression in non-transfected (A) and human CIS transfected (B) CHO whole cell lysates.Application
Western blot analysis of CIS expression in mouse liver tissue extract.| Product Name | CIS Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-80 mapping at the N-terminus of cytokine-inducible SH2 protein (CIS) of human origin |
| Modification Notes | Src homology (SH2) domains are noncatalytic sequences that are conserved among a number of cytoplasmic signaling proteins. These signaling proteins are directly regulated by receptor tyrosine kinases and control the activation of mitogenic signal transduction pathways by such receptors. For instance, ligand-induced activation of the EGF and PDGF receptors induces dimerization, triggers receptor autophosphorylation on tyrosine residues and results in the binding of a number of cytoplasmic SH2 domain proteins such as PLC-g1, Ras GAP and PI 3 kinase p85 to the activated receptors. The Shc gene encodes three proteins with a single SH2 domain but no identifiable catalytic domain. CIS, cytokine-inducible SH2-containing protein, is a 267 amino acid protein with a single 96 amino acid SH2 domain that associates the tyrosine-phosphorylated beta chain of the IL-3 receptor with the tyrosine-phosphorylated Epo receptor. CIS was initially described as an immediate early cytokine-responsive gene and appears to be a unique regulator of cytokine signaling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CIS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-80 mapping at the N-terminus of cytokine-inducible SH2 protein (CIS) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-80 mapping at the N-terminus of cytokine-inducible SH2 protein (CIS) of human origin |
| Entrez Gene | 1154 |
|---|
Application
Western blot analysis of CIS expression in non-transfected: (A) and human CIS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CIS expression in non-transfected (A) and human CIS transfected (B) CHO whole cell lysates.
Application
Western blot analysis of CIS expression in mouse liver tissue extract.| Application Notes | recommended for detection of CIS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
