Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing nuclear staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular and lymphoid cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Sp1 expression in Jurkat nuclear extract.Application
Western blot analysis of Sp1 expression in K-562 (A) and A-431 (B) whole cell lysates.| Product Name | Sp1 Antibody (H-225) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 121-345 mapping near the N-terminus of Sp1 of human origin |
| Modification Notes | Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A) (7), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Sp1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 121-345 mapping near the N-terminus of Sp1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 121-345 mapping near the N-terminus of Sp1 of human origin |
| Entrez Gene | 6667 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing nuclear staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular and lymphoid cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Sp1 expression in Jurkat nuclear extract.
Application
Western blot analysis of Sp1 expression in K-562 (A) and A-431 (B) whole cell lysates.| Application Notes | recommended for detection of Sp1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
