Application
Western blot analysis of NKG2-D expression in A549 whole cell lysate.Application
Western blot analysis of NKG2-D expression in TK-1 (A) and WEHI-231 (B) whole cell lysates.Application
Western blot analysis of NKG2-D expression in K-562 whole cell lysate.Application
Western blot analysis of NKG2-D expression in mouse spleen tissue extract.| Product Name | NKG2-D Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 108-172 mapping within an internal region of NKG2-D of human origin |
| Modification Notes | The activity of natural killer (NK) cells is regulated by members of multiple receptor families that recognize class I MHC molecules, such as the killer cell inhibitory receptor/leukocyte immunoglobulin-like receptor (KIR/LIR) family and the C-type lectin superfamily. The KIR/LIR family includes p91A (also designated pp130 or PIR-B, for paired Immunoglobulin-like receptor-B) and p91B (also designated PIR-A). p91A acts as an inhibitory receptor through interactions with SHP-1, whereas p91B acts as an activating receptor. CD94, NKG2 and Ly-49 are members of the C-type lectin superfamily of type II membrane glycoproteins. CD94 forms heterodimers with NKG2 isoforms on the surface of NK cells, whereas Ly-49 isoforms form homodimers. NKG2-D, expressed on NK cells, ©∂ T cells, and CD8+ å∫ T cells, is a receptor for the stress inducible protein MICA, an antigen frequently expressed in epithelial tumors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NKG2-D of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 108-172 mapping within an internal region of NKG2-D of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 108-172 mapping within an internal region of NKG2-D of human origin |
| Entrez Gene | 22914 |
|---|
Application
Western blot analysis of NKG2-D expression in A549 whole cell lysate.
Application
Western blot analysis of NKG2-D expression in TK-1 (A) and WEHI-231 (B) whole cell lysates.
Application
Western blot analysis of NKG2-D expression in K-562 whole cell lysate.
Application
Western blot analysis of NKG2-D expression in mouse spleen tissue extract.| Application Notes | recommended for detection of NKG2-D of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
