Application
Western blot analysis of Wnt-1 expression in WI 38 whole cell lysate.| Product Name | Wnt-1 Antibody (H-89) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-89 of Wnt-1 of human origin |
| Modification Notes | The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis (1). The Wnt family has two functional classes according to their biological activities; Wnts that signal through a Wnt-1/wingless pathway by stabilizing cytoplasmic beta-catenin, and Wnts that stimulate intracellular Ca2+ release and activate two kinases, CamKII and PKC, in a G protein-dependent manner (1,2). Cytoplasmic relay components transduce Wnt-induced, frizzled-mediated signals to beta-catenin, which translocates to the nucleus and forms a complex with T-cell factor to then activate transcription of Wnt target genes (3). Some of the target genes influence the promotion of tumor progression, invasion, and metastasis, such as c-Myc, cyclin D1, c-jun, fra-1, and u-PAR (3). The human Wnt-1 gene clusters with Wnt-10b in the chromosome 12q13 region (4,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Wnt-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-89 of Wnt-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-89 of Wnt-1 of human origin |
| Entrez Gene | 7471 |
|---|
Application
Western blot analysis of Wnt-1 expression in WI 38 whole cell lysate.| Application Notes | recommended for detection of Wnt-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
