Application
Western blot analysis of Ciao 1 expression in non-transfected: (A) and human Ciao 1 transfected: (B) 293T whole cell lysates.| Product Name | Ciao 1 Antibody (H-339) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-339 representing full length Ciao 1 of human origin |
| Modification Notes | The Wilms’ tumor suppressor protein, WT1, contains a zinc finger domain and is capable of both activating or repressing transcription, depending on cell type and promotor context (1). A number of proteins, including various tumor suppressors, have been shown to interact with WT1. Interaction of WT1 with p53 results in increased p53 stability, and inhibits the ability of p53 to induce apoptosis (2). Par-4, a transcriptional repressor, is also known to bind WT1 (3). Ciao 1, a member of the WD40 family of proteins, specifically interacts with WT1, resulting in a decrease in WT1 mediated transcriptional activation. Ciao 1 does not inhibit binding by causing a conforma-tional change or by interfering with the activation domain of WT1 (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ciao 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-339 representing full length Ciao 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-339 representing full length Ciao 1 of human origin |
| Entrez Gene | 9391 |
|---|
Application
Western blot analysis of Ciao 1 expression in non-transfected: (A) and human Ciao 1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ciao 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
