Application
Western blot analysis of Lck BP-1 expression in non-transfected: (A) and human Lck BP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Lck BP-1 expression in non-transfected 293T: (A), human Lck BP-1 transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of Lck BP-1 expression in Ramos (A), CCRF-CEM (B) and BJAB (C) whole cell lysates.| Product Name | Lck BP-1 Antibody (H-135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 352-486 mapping at the C-terminus of Lck BP-1 of human origin |
| Modification Notes | A total of eight membrane-associated tyrosine protein kinases have been identified within the Src gene family (1). These include c-Src, c-Yes, Fyn, Lck, Hck, Lyn, Blk and c-Fgr. The major translational product of the human Lck gene is a lymphocyte-specific tyrosine kinase designated pp56Lck. This is a membrane-associated molecule, most likely via covalently associated myristate at the amino terminus (2,3). The Lck gene has been shown to undergo rearrangement and overexpression in some murine lymphomas (3,4). In human studies, it has been demonstrated that the Lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas (5). A novel Lck signaling intermediate, designated Lck BP-1, associates directly with the Lck SH3 domian via two proline-rich regions (6). Lck BP-1 also contains four tandem 37 amino acid repeats that form a putative helix-loop-helix DNA binding motif (6). Immunoprecipitation studies have shown that Lck BP-1 will co-immunoprecipitate with Lck from T cell lysates. Lck BP-1 is tyrosine phosphorylated in T cells subsequent to TCR activation (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Lck BP-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 352-486 mapping at the C-terminus of Lck BP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 352-486 mapping at the C-terminus of Lck BP-1 of human origin |
| Entrez Gene | 3059 |
|---|
Application
Western blot analysis of Lck BP-1 expression in non-transfected: (A) and human Lck BP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Lck BP-1 expression in non-transfected 293T: (A), human Lck BP-1 transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of Lck BP-1 expression in Ramos (A), CCRF-CEM (B) and BJAB (C) whole cell lysates.| Application Notes | recommended for detection of Lck BP-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
