Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic and nuclear staining of hematopoietic cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytplasmic localization.Application
Western blot analysis of cyclin B2 expression in non-transfected 293T: (A), mouse cyclin B2 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing membrane and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of cyclin B2 expression in K-562 (A) and F9 (B) whole cell lysates.| Product Name | cyclin B2 Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-105 of cyclin B2 of human origin |
| Modification Notes | In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of cyclin B2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-105 of cyclin B2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-105 of cyclin B2 of human origin |
| Entrez Gene | 9133 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic and nuclear staining of hematopoietic cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytplasmic localization.
Application
Western blot analysis of cyclin B2 expression in non-transfected 293T: (A), mouse cyclin B2 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing membrane and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of cyclin B2 expression in K-562 (A) and F9 (B) whole cell lysates.| Application Notes | recommended for detection of cyclin B2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
