Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear, cytoplasmic and membrane staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TLR-5 expression in THP-1 (A) and NAMALWA (B) whole cell lysates.| Product Name | TLR5 Antibody (H-127) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 154-280 mapping near the N-terminus of TLR5 (Toll-like receptor 5) of human origin |
| Modification Notes | Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities and designated toll-like receptors (TLR) (1,2). Toll receptors are involved in mediating dorsoventral polarization in the developing Drosophila embryo and also participate in the host immunity. The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction (3). TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain (4). TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses (5,6). TLR5 specifically participates in the innate immune response to microbial agents. TLR5 is highly expressed in ovary and in peripheral blood leukocytes, most abundantly in monocytes and, to a lesser extent, in prostate and testis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TLR5 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 154-280 mapping near the N-terminus of TLR5 (Toll-like receptor 5) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 154-280 mapping near the N-terminus of TLR5 (Toll-like receptor 5) of human origin |
| Entrez Gene | 7100 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear, cytoplasmic and membrane staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TLR-5 expression in THP-1 (A) and NAMALWA (B) whole cell lysates.| Application Notes | recommended for detection of TLR5 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
