Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.Application
Western blot analysis of CaMKIIγ expression in non-transfected: (A) and human CaMKIIγ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CaMKII expression in mouse brain tissue extract.Application
Western blot analysis of CaMKII expression in mouse brain tissue extractApplication
Western blot analysis of CaMKIIδ expression in non-transfected: (A) and human CaMKIIδ transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing cytoplasmic localization.| Product Name | CaMKII Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CaMKIIα of human origin |
| Modification Notes | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated å, ∫, © and ∂, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of α, β, γ and δ subunits of CaMKII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CaMKII&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CaMKIIα of human origin |
| Entrez Gene | 815 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
Application
Western blot analysis of CaMKIIγ expression in non-transfected: (A) and human CaMKIIγ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CaMKII expression in mouse brain tissue extract.
Application
Western blot analysis of CaMKII expression in mouse brain tissue extract
Application
Western blot analysis of CaMKIIδ expression in non-transfected: (A) and human CaMKIIδ transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing cytoplasmic localization.| Application Notes | recommended for detection of α, β, γ and δ subunits of CaMKII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
